Vibrio harveyi
About
This bacteria is an advantageous good engineerable chassis because it can respond to several quorum sensing molecule.
References:
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1578700/
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3285556/
Growth Characteristics
As Vibrio harveyi is a bacteria found in sea water, it can not survive without salt in the medium. LB can be enough but Luria Marine is better.
Transformation Protocols
- Triparental conjugation: https://pub.uni-bielefeld.de/publication/1659456
BioBrick Parts
- LacI-mRFP: http://parts.igem.org/Part:BBa_J04450
Whole Cell Metabolism SBML Model
Vectors
- pRK2073 helper plasmid
- pBBR1MCS5 - genta conjugative plasmid:
Acknowledgements
This entry was curated by iGEM 2017 team INSA-UPS France