Saccharomyces cerevisiae

eukaryotic facultative-anaerobic fungal yeast

Growth Characteristics

• Temperature: 25°C to 30°C
• Atmosphere: Typical aerobic

References:

1. ATCC

Culture Sources

1. ATCC

Maintenance Protocols

1. Growth and Maintnenance protocol: https://link.springer.com/protocol/10.1385%2F1-59259-210-4%3A009

Transformation Protocols

1. High Efficiency Transformation: https://openwetware.org/wiki/High_Efficiency_Transformation

Other Protocols

1. Cell Cycle Arrest: https://openwetware.org/wiki/Cell_Cycle_Arrest

2. Assaying Mating: https://openwetware.org/wiki/Assaying_mating

3. DNA prep: https://openwetware.org/wiki/Yeast_DNA_Prep

4. Colony PCR: https://openwetware.org/wiki/Yeast_Colony_PCR

BioBrick Parts

1. BBa_K401001

2. BBa_K801000

3. BBa_K2082208

4. BBa_K2118002

Vectors

1. pYAC4 (Burke87)

2. pJS97 / pJS98 plasmids (Shero91)(https://openwetware.org/wiki/Yeast_artificial_chromosomes)

3. pCGS966 (Smith90, Smith92, Moir93)

4. pRML1 / pRML2 (Spencer93)

Whole Cell Metabolism SBML Models

• iMM904

• iND750

• ATCC 204508 / S288c

Acknowledgements

Data curated by iGEM 2017 team IIT Madras: igem@smail.iitm.ac.in