Pichia pastoris

eukaryotic facultative-anaerobic fungal yeast

Culture Sources

1. ATCC 28485

2. NCYC 175

3. YPD

Maintenance Protocols

1. Temperature: 24°C to 26°C Atmosphere: Typical aerobic growth media: YEPD, YM agar or broth, Emmons' modification of Sabouraud's agar: https://www.atcc.org/~/ps/28485.ashx

Transformation Protocols

1. Pichia Yeast Transformation protocol - iGEM 2012: http://2012.igem.org/wiki/images/0/02/Pichia_Yeast_Transformation_protocol.pdf

2. Condensed protocol for competent cell preparation and transformation of the methylotrophic yeast Pichia pastoris: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2504082/

3. High efficiency transformation by electroporation of Pichia pastoris: https://www.biotechniques.com/multimedia/archive/00003/BTN_A_05381BM04_O_3873a.pdf

BioBrick Parts

1. pPICZa

2. Leucrocine I AMP + afactor

3. cOT2 AMP + afactor

4. D-NY15 AMP + afactor

5. AOX1 promoter

6. afactor secretion signal

7. BBa_I764001

8. BBa_K863203

9. BBa_K805010

Whole Cell Metabolism SBML Models

• iLC915
• iPP668 / PpaMBEL1254 / iLC915
• iPP668

Vectors

1. pPICZa
2. pPIC9K

Acknowledgements

This entry was curated by iGEM 2017 teams IISc-Bangalore and INSA-UPS France