Lactococcus lactis

prokaryotic facultative-anaerobic gram-positive lactose-fermenting

Maintenance Protocols

1. Media and growth conditions for Lactococcus lactis :
   http://classrooms.tacoma.k12.wa.us/wilson/vrehberg/documents/download/agar_multitype_basic_protocols.pdf?id=145903

Transformation Protocols

1. Efficient transformation:
   http://onlinelibrary.wiley.com/doi/10.1002/jobm.200610297/pdf

2. Electrotransformation of Lactococcus lactis:
   https://www.nature.com/protocolexchange/protocols/287#/procedure

Other Protocols

1. VBEx (Vector backbone exchange) procedure:
   https://www.nature.com/protocolexchange/protocols/287#/procedure

BioBrick Parts

1. BBa_K137009

2. BBa_K137011

3. BBa_K137005

4. BBa_K137006

Vectors

1. pSpeedET

2. pSEUDO

Whole Cell Metabolism SBML Models

• Cremoris NZ9000

Acknowledgements

Data curated by iGEM 2017 team IIT Madras: igem@smail.iitm.ac.in