Fusarium solani

eukaryotic fungal filamentous fungus aerobic facultative-anaerobic

Growth Characteristics

• Temperature: 24°C - 30°C
• Optimal Temperature: 27°C
• Growth period: 7 days
• Atmosphere: Typical aerobic
• Growth medium: Potato Dextrose (PDA/PDB)
• Storage conditions: As Glycerol stock at -80°C

Special Characteristics:

1. Capable to adapt to many different environments by virtue of their genetic plasticity and metabolic diversity.

Potential Uses:

1. Potential to produce Camptothecin and 10-hydroxycamptothecin
2. Cyclosporin A production
3. Capable of BaP degradation

Culture Sources

1. ATCC
2. MTCC

Transformation Protocols

1. Electroporation: https://academic.oup.com/nar/article-abstract/18/22/6737/1054876?redirectedFrom=fulltext
2. High efficiency transformation: https://link.springer.com/content/pdf/10.1007%2FBF00351656.pdf

BioBrick Parts

1. BBa_K3163004

Vectors

1. pBHt2
2. pETM2
3. pAN7-1, pGC1-1, pRC13, pRCOS-1
4. pDC1
5. pKBY2

References:

1. https://www.atcc.org/Products/All/60860.aspx#culturemethod
2. https://www.sciencedirect.com/science/article/abs/pii/S0031942209004208
3. https://www.sciencedirect.com/science/article/pii/S0960852415000073
4. https://fungi.ensembl.org/Fusarium_solani/Info/Index
5. https://www.ncbi.nlm.nih.gov/pubmed/29392603

Acknowledgements

Data curated by iGEM 2017 team IIT Madras: igem@smail.iitm.ac.in