Escherichia coli

prokaryotic facultative-anaerobic gram-negative

Growth Characteristics

• Optimum pH: between 4.4 and 9.7 (7 is the best)
• Doubling Time: 20 min
• Optimum Temperature: 37 degrees

Reference

https://www.atcc.org/products/all/25922.aspx

Culture Sources

1. ATCC

Maintenance protocols

1. Basic growth: http://www.sigmaaldrich.com/technical-documents/protocols/biology/microbial-growth.html

Transformation protocols

1. Addgene protocol: https://www.addgene.org/protocols/bacterial-transformation/

BioBrick parts

1. BBa_J23055
2. BBa_J23056

Vectors

[1] pBR322

Whole Cell Metabolism SBML models

• iECD_1391
• iB21_1397
• K12

Other online resources

• Ecoliwiki
• EcoCyc
• PortEco

Acknowledgements

Data curated by iGEM 2017 team IIT Madras: igem@smail.iitm.ac.in