Bacillus subtilis

prokaryotic facultative-anaerobic gram-positive endosporulating

Culture sources

  1. ATCC
  2. Bacillus Genetic Stock Centre

Growth Media

  1. LB broth: http://2014.igem.org/wiki/images/5/59/LMU_Munich14_Media.pdf
  2. Difco Sporulation medium: http://2014.igem.org/wiki/images/5/59/LMU_Munich14_Media.pdf
  3. Chemically defined medium: https://file.scirp.org/pdf/AiM_2014061709514537.pdf
  4. Modified Chemically Defined Media: http://2014.igem.org/wiki/images/5/59/LMU_Munich14_Media.pdf

Transformation Protocols

  1. Efficient Transformation: https://link.springer.com/article/10.1007%2Fs00253-012-3987-2
  2. Requirements for transformation: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC279084/pdf/jbacter00480-0093.pdf
  3. How To Bacillus subtilis: http://2016.igem.org/wiki/images/7/74/T--UBonn_HBRS--How-To-Bacillus-Subtilis.pdf

Other Protocols

  1. Cell disruption and extraction of RNA: http://subtiwiki.uni-goettingen.de/wiki/images/8/82/SOP-RNA.pdf
  2. Transposon mutagenesis: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3264109/

BioBrick Parts

  1. BBa_I742123
  2. pBS1C
  3. pBS2E
  4. pBs4S
  5. Episomal Vector with Constitutive GFP
  6. Shuttle Vector for Chromosomal Integration
  7. BBa_K1065204
  8. Integration vector for Bacillus subtilis derived from pSac-Cm
  9. pSBbs4E
  10. pSBBs1C-lacZ
  11. pSBBs4S
  12. pSBBs1C
  13. pSBBs4S-P
  14. pSBBs3C-luxABCDE
  15. pSBBs2E

Vectors

  1. PAC5
  2. PAC6
  3. PAC7
  4. pBaSysBioII

Whole-Cell Metabolism Model

Other online resources

  1. SubtiWiki: http://subtiwiki.uni-goettingen.de/

References

Acknowledgements

Data curated by iGEM 2017 team IIT Madras: igem@smail.iitm.ac.in