Acinetobacter baylyi

prokaryotic aerobic gram-negative naturally-competent

Applications

It is a non-pathogenic, nutritionally versatile, naturally competent organism that can be used in various applications.

Strains

• ADP1
• ADP1-ISx

Life Cycle

• Doubling Time: 30-40 min
• Optimal Temperature: 30°C
• Incubation Period: 1-2 days

Protocols

http://barricklab.org/twiki/bin/view/Lab/ProtocolList#Acinetobacter_baylyi_ADP1

Vectors

• pBAV1K

BioBrick Parts

• BBa_K2146001: A. baylyi origin of replication
• BBa_K2146003: A. baylyi shuttle vector with spectinomycin resistance.
• BBa_K2146004: A. baylyi shuttle vector with cefotaxime resistance.
• BBa_K2146005: A. baylyi shuttle vector with amikacin resistance.
• BBa_K2146006: A. baylyi shuttle vector with cefotaxime resistance, containing reporter gene gusA

Whole Cell SBML Model

• ADP1

Genome Sequence

https://www.ncbi.nlm.nih.gov/nuccore/CR543861.1

Acknowledgements

Data curated by iGEM 2018 team IIT Madras: igem@smail.iitm.ac.in