Acidithiobacillus ferrooxidans

prokaryotic facultative-anaerobic chemolithoautotrophic acidophilic metal-solubilizing gram-negative

Culture Sources

1. ATCC 23270

Maintenance Protocols

1. ATCC Medium 2039

2. DSMZ Medium 70

3. DSMZ Medium 71

4. General Information

Transformation Protocols

1. Electrotransformation of Thiobacillus ferrooxidans with Plasmids:
   https://www.ncbi.nlm.nih.gov/pmc/articles/PMC207636/pdf/jbacter00086-0309.pdf

Other Protocols

1. Metabolomic study of Chilean biomining bacteria Acidithiobacillus ferrooxidans strain Wenelen and Acidithiobacillus thiooxidans strain Licanantay:
   https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3548112/

2. Comparative Genomics of the Extreme Acidophile *Acidithiobacillus thiooxidans* Reveals Intraspecific Divergence and Niche Adaptation:
   https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5000751/

Whole Cell Metabolism SBML model

• ATCC 23270 / DSM 14882 / NCIB 8455

BioBrick Parts

1. Iron oxidase generator

2. Inverter with Ferric Uptake Regulator & LacI. Activates gene expression in presence of iron

3. Promoter + Inverter with Ferric Uptake Regulator & LacI. Activates gene expression in presence of Fe

4. Part designed for verification and characterization of the FUR/LacI inverter

5. Tetrathionate Hydrolase (TetH) CDS with a RBS for overexpression in *Acidithiobacillus ferrooxidans*

6. Heat Shock Promoter

7. Heat shock promoter with RBS and RFP

8. Part designed for verification and characterization of the heat the shock promoter and Inverter

9. Overexpression of TetH with heat shock promoter and Inverter

Acknowledgements

Data curated by iGEM 2017 team IIT Madras: igem@smail.iitm.ac.in